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rabbit anti-gdf15 antibody  (Servicebio Inc)

 
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    Servicebio Inc rabbit anti-gdf15 antibody
    Rabbit Anti Gdf15 Antibody, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti-gdf15+antibody/rabbit+anti+gdf15+antibody/ppr0712718-96-27-31
    Average 90 stars, based on 1 article reviews
    rabbit anti-gdf15 antibody - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: JQ1 inhibits the proliferation of thyroid cancer TPC-1 cells by regulating the ferroptosis genes ALOX5 and GDF15
    Article Snippet: A rabbit secondary antibody detection kit (PV9001, ZSGB Bio, Beijing) was used for primary antibodies using 1:2,000 diluted rabbit anti-ALOX5 antibody (bs3818R, Bioss, Beijing) and 1:500 diluted rabbit anti-GDF15 antibody (GB111330, Servicebio, Wuhan) and a 3,3 N-diaminobenzidine tetrahydrochloride (DAB) colour development kit (ZLI-9018, ZSGB Bio, Beijing).



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    Image Search Results


    GDF15 preserves mitochondrial homeostasis in LPS-stimulated macrophages through dual regulation of SMAD7 and PKM2 pathways. (A) HIF-1α and SMAD7 expression in RAW264.7 macrophages across conditions: Untreated, LPS, LPS with rAAV8-mGdf15 overexpression (LPS+GDF15), and LPS with GDF15 knockdown (si-GDF15). β-actin: loading control.(HIF-1α suppression and SMAD7 induction by GDF15.) (B) Cytosolic and nuclear PKM2 protein levels. Lamin B1 (nuclear) and α-tubulin (cytosolic) markers validate fractionation efficiency. Study groups and individual replicates are identified in the figure key.(PKM2 subcellular redistribution modulated by GDF15.) (C) Immunofluorescence of PKM2 (red) and nuclei (DAPI, blue). Arrows indicate nuclear PKM2 accumulation. Scale bar: 15 μm.(Nuclear PKM2 enrichment upon LPS challenge mitigated by GDF15 and exacerbated by GDF15 knockdown.).

    Journal: Frontiers in Immunology

    Article Title: GDF15 orchestrates mitochondrial-immune crosstalk via SMAD7-HIF-1α-PKM2 cascade to attenuate septic liver injury

    doi: 10.3389/fimmu.2025.1712741

    Figure Lengend Snippet: GDF15 preserves mitochondrial homeostasis in LPS-stimulated macrophages through dual regulation of SMAD7 and PKM2 pathways. (A) HIF-1α and SMAD7 expression in RAW264.7 macrophages across conditions: Untreated, LPS, LPS with rAAV8-mGdf15 overexpression (LPS+GDF15), and LPS with GDF15 knockdown (si-GDF15). β-actin: loading control.(HIF-1α suppression and SMAD7 induction by GDF15.) (B) Cytosolic and nuclear PKM2 protein levels. Lamin B1 (nuclear) and α-tubulin (cytosolic) markers validate fractionation efficiency. Study groups and individual replicates are identified in the figure key.(PKM2 subcellular redistribution modulated by GDF15.) (C) Immunofluorescence of PKM2 (red) and nuclei (DAPI, blue). Arrows indicate nuclear PKM2 accumulation. Scale bar: 15 μm.(Nuclear PKM2 enrichment upon LPS challenge mitigated by GDF15 and exacerbated by GDF15 knockdown.).

    Article Snippet: Proteins (30 μg/lane) were separated on 10% SDS-PAGE gels, transferred to PVDF membranes (Millipore, IPVH00010), and probed with the following primary antibodies: rabbit anti-mouse UQCRC1 (Proteintech, Cat. No. 21705-1-AP, 1:1000), rabbit anti-mouse GDF15 (Proteintech, Cat. No. 27455-1-AP, 1:1000), rabbit anti-mouse HIF-1α (Proteintech, Cat. No. 20960-1-AP, 1:1000), rabbit anti-mouse SMAD7 (Proteintech, Cat. No. 725840-1-AP, 1:1000), mouse anti-mouse PKM2 (Proteintech, Cat. No. 60268-1-Ig, 1:1000), rabbit anti-mouse H3 (Proteintech, Cat. No. 17168-1-AP, 1:5000) and mouse anti-mouse β-actin (Proteintech, Cat. No. 66009-1-Ig, 1:5000).

    Techniques: Expressing, Over Expression, Knockdown, Control, Fractionation, Immunofluorescence

    HIF-1α and PKM2 are critical effectors of GDF15-driven mitochondrial protection and anti-inflammatory responses. (A) HIF-1α inhibition by BAY 87-2243 (5 μM, 24 h). β-actin: loading control.(Pharmacological HIF-1α blockade.) (B) PKM2 inhibition by Shikonin (2 μM, 24 h). β-actin: loading control.(PKM2 activity suppression.) (C) UQCRC1 recovery in LPS-injured macrophages treated with: GDF15 overexpression, HIF-1α inhibitor (BAY), or PKM2 inhibitor (Shikonin). β-actin: loading control.(Mitochondrial complex III rescue via HIF-1α/PKM2 inhibition mirrors GDF15 effects.) (D) Inflammatory (TNF-α, IL-6) and metabolic (lactate) markers in cell supernatant (n = 5). Study groups and individual replicates are identified in the figure key. ***p < 0.001.(HIF-1α/PKM2 targeting replicates GDF15-mediated anti-inflammatory and metabolic homeostasis.) (E) UQCRC1 expression under GDF15 loss-of-function: si-GDF15 alone vs. combined with BAY 87–2243 or Shikonin. β-actin: loading control. Study groups and individual replicates are identified in the figure key.(Mitochondrial rescue in GDF15-deficient macrophages requires HIF-1α/PKM2 inhibition.) (F) Supernatant cytokines and lactate in si-GDF15 macrophages with/without inhibitors (n = 5). ***p < 0.001. (Inflammation reversal in GDF15-knockdown macrophages depends on HIF-1α/PKM2 blockade.).

    Journal: Frontiers in Immunology

    Article Title: GDF15 orchestrates mitochondrial-immune crosstalk via SMAD7-HIF-1α-PKM2 cascade to attenuate septic liver injury

    doi: 10.3389/fimmu.2025.1712741

    Figure Lengend Snippet: HIF-1α and PKM2 are critical effectors of GDF15-driven mitochondrial protection and anti-inflammatory responses. (A) HIF-1α inhibition by BAY 87-2243 (5 μM, 24 h). β-actin: loading control.(Pharmacological HIF-1α blockade.) (B) PKM2 inhibition by Shikonin (2 μM, 24 h). β-actin: loading control.(PKM2 activity suppression.) (C) UQCRC1 recovery in LPS-injured macrophages treated with: GDF15 overexpression, HIF-1α inhibitor (BAY), or PKM2 inhibitor (Shikonin). β-actin: loading control.(Mitochondrial complex III rescue via HIF-1α/PKM2 inhibition mirrors GDF15 effects.) (D) Inflammatory (TNF-α, IL-6) and metabolic (lactate) markers in cell supernatant (n = 5). Study groups and individual replicates are identified in the figure key. ***p < 0.001.(HIF-1α/PKM2 targeting replicates GDF15-mediated anti-inflammatory and metabolic homeostasis.) (E) UQCRC1 expression under GDF15 loss-of-function: si-GDF15 alone vs. combined with BAY 87–2243 or Shikonin. β-actin: loading control. Study groups and individual replicates are identified in the figure key.(Mitochondrial rescue in GDF15-deficient macrophages requires HIF-1α/PKM2 inhibition.) (F) Supernatant cytokines and lactate in si-GDF15 macrophages with/without inhibitors (n = 5). ***p < 0.001. (Inflammation reversal in GDF15-knockdown macrophages depends on HIF-1α/PKM2 blockade.).

    Article Snippet: Proteins (30 μg/lane) were separated on 10% SDS-PAGE gels, transferred to PVDF membranes (Millipore, IPVH00010), and probed with the following primary antibodies: rabbit anti-mouse UQCRC1 (Proteintech, Cat. No. 21705-1-AP, 1:1000), rabbit anti-mouse GDF15 (Proteintech, Cat. No. 27455-1-AP, 1:1000), rabbit anti-mouse HIF-1α (Proteintech, Cat. No. 20960-1-AP, 1:1000), rabbit anti-mouse SMAD7 (Proteintech, Cat. No. 725840-1-AP, 1:1000), mouse anti-mouse PKM2 (Proteintech, Cat. No. 60268-1-Ig, 1:1000), rabbit anti-mouse H3 (Proteintech, Cat. No. 17168-1-AP, 1:5000) and mouse anti-mouse β-actin (Proteintech, Cat. No. 66009-1-Ig, 1:5000).

    Techniques: Inhibition, Control, Activity Assay, Over Expression, Expressing, Knockdown

    SMAD7 activation suppresses HIF-1α to mediate GDF15-dependent mitochondrial protection in LPS-challenged macrophages. (A) Pharmacological SMAD7 activation by Asiaticoside (20 μM, 48 h). β-actin: loading control. (B) HIF-1α expression under LPS challenge: LPS alone, LPS + AVV-GDF15, or LPS + SMAD7 activation (Asiaticoside). β-actin: loading control. (C) HIF-1α modulation across conditions: LPS, LPS + si-GDF15, LPS + Asiaticoside, or LPS + si-GDF15 + Asiaticoside. β-actin: loading control.

    Journal: Frontiers in Immunology

    Article Title: GDF15 orchestrates mitochondrial-immune crosstalk via SMAD7-HIF-1α-PKM2 cascade to attenuate septic liver injury

    doi: 10.3389/fimmu.2025.1712741

    Figure Lengend Snippet: SMAD7 activation suppresses HIF-1α to mediate GDF15-dependent mitochondrial protection in LPS-challenged macrophages. (A) Pharmacological SMAD7 activation by Asiaticoside (20 μM, 48 h). β-actin: loading control. (B) HIF-1α expression under LPS challenge: LPS alone, LPS + AVV-GDF15, or LPS + SMAD7 activation (Asiaticoside). β-actin: loading control. (C) HIF-1α modulation across conditions: LPS, LPS + si-GDF15, LPS + Asiaticoside, or LPS + si-GDF15 + Asiaticoside. β-actin: loading control.

    Article Snippet: Proteins (30 μg/lane) were separated on 10% SDS-PAGE gels, transferred to PVDF membranes (Millipore, IPVH00010), and probed with the following primary antibodies: rabbit anti-mouse UQCRC1 (Proteintech, Cat. No. 21705-1-AP, 1:1000), rabbit anti-mouse GDF15 (Proteintech, Cat. No. 27455-1-AP, 1:1000), rabbit anti-mouse HIF-1α (Proteintech, Cat. No. 20960-1-AP, 1:1000), rabbit anti-mouse SMAD7 (Proteintech, Cat. No. 725840-1-AP, 1:1000), mouse anti-mouse PKM2 (Proteintech, Cat. No. 60268-1-Ig, 1:1000), rabbit anti-mouse H3 (Proteintech, Cat. No. 17168-1-AP, 1:5000) and mouse anti-mouse β-actin (Proteintech, Cat. No. 66009-1-Ig, 1:5000).

    Techniques: Activation Assay, Control, Expressing

    Fig. 2. Growth differentiation factor 15 (GDF15) had cardioprotective effects in HFpEF. (A) Silencing GDF15 further exacerbates

    Journal: Frontiers in Bioscience-Landmark

    Article Title: Growth Differentiation Factor 15 Inhibits Cardiac Fibrosis, Oxidative Stress, Inflammation, and Apoptosis in a Rat Model of Heart Failure with Preserved Ejection Fraction

    doi: 10.31083/fbl26857

    Figure Lengend Snippet: Fig. 2. Growth differentiation factor 15 (GDF15) had cardioprotective effects in HFpEF. (A) Silencing GDF15 further exacerbates

    Article Snippet: The primary antibodies utilized included rabbit anti-GDF15 antibody (1:2000; 27455-1-AP; Proteintech; Wuhan, China), mouse anti-NTproBNP antibody (1:1000; ab13115; abcam; Cambridge, UK), rabbit anti-Collagen I antibody (1:1000; 14695-1-AP; Proteintech; Wuhan, China), rabbit anti-Collagen III antibody (1:300; 22734-1-AP; Proteintech; Wuhan, China), rabbit anti-α-smooth muscle actin (α-SMA) antibody (1:1000; 14395-1-AP; Proteintech; Wuhan, China), rabbit anti-Myeloperoxidase (MPO) antibody (1:1000; 22225- 1-AP; Proteintech; Wuhan, China), rabbit anti-oxidized low-density lipoprotein (ox-LDL) antibody (1:500; Ys1698R; YaJi Biotechnology Co., Ltd; Shanghai, China), rabbit anti-Interleukin-8 (IL8) antibody (1:500; 27095-1- AP; Proteintech; Wuhan, China), rabbit anti-Interleukin-6 (IL6) antibody (1:500; 21865-1-AP; Proteintech; Wuhan, China), rabbit anti-Tumor Necrosis Factor α (TNFα) antibody (1:500; 17590-1-AP; Proteintech; Wuhan, China), rabbit anti-Interleukin-1 beta (IL-1β) antibody (1:500; 16806-1-AP; Proteintech; Wuhan, China), rabbit antiCleaved Caspase-3 antibody (1:1000; 66470-2-Ig; Proteintech; Wuhan, China), and rabbit anti-BCL2-associated X (Bax) antibody (1:2000; 50599-2-Ig; Proteintech; Wuhan, China).

    Techniques:

    Fig. 3. GDF15 inhibited cardiac fibrosis in HFpEF. (A) Masson’s trichrome staining for collagen deposition in the cardiac tissue

    Journal: Frontiers in Bioscience-Landmark

    Article Title: Growth Differentiation Factor 15 Inhibits Cardiac Fibrosis, Oxidative Stress, Inflammation, and Apoptosis in a Rat Model of Heart Failure with Preserved Ejection Fraction

    doi: 10.31083/fbl26857

    Figure Lengend Snippet: Fig. 3. GDF15 inhibited cardiac fibrosis in HFpEF. (A) Masson’s trichrome staining for collagen deposition in the cardiac tissue

    Article Snippet: The primary antibodies utilized included rabbit anti-GDF15 antibody (1:2000; 27455-1-AP; Proteintech; Wuhan, China), mouse anti-NTproBNP antibody (1:1000; ab13115; abcam; Cambridge, UK), rabbit anti-Collagen I antibody (1:1000; 14695-1-AP; Proteintech; Wuhan, China), rabbit anti-Collagen III antibody (1:300; 22734-1-AP; Proteintech; Wuhan, China), rabbit anti-α-smooth muscle actin (α-SMA) antibody (1:1000; 14395-1-AP; Proteintech; Wuhan, China), rabbit anti-Myeloperoxidase (MPO) antibody (1:1000; 22225- 1-AP; Proteintech; Wuhan, China), rabbit anti-oxidized low-density lipoprotein (ox-LDL) antibody (1:500; Ys1698R; YaJi Biotechnology Co., Ltd; Shanghai, China), rabbit anti-Interleukin-8 (IL8) antibody (1:500; 27095-1- AP; Proteintech; Wuhan, China), rabbit anti-Interleukin-6 (IL6) antibody (1:500; 21865-1-AP; Proteintech; Wuhan, China), rabbit anti-Tumor Necrosis Factor α (TNFα) antibody (1:500; 17590-1-AP; Proteintech; Wuhan, China), rabbit anti-Interleukin-1 beta (IL-1β) antibody (1:500; 16806-1-AP; Proteintech; Wuhan, China), rabbit antiCleaved Caspase-3 antibody (1:1000; 66470-2-Ig; Proteintech; Wuhan, China), and rabbit anti-BCL2-associated X (Bax) antibody (1:2000; 50599-2-Ig; Proteintech; Wuhan, China).

    Techniques: Staining

    Fig. 4. GDF15 decreased oxidative stress in HFpEF. Expression of oxidative stress-associated biomarkers, Myeloperoxidase (MPO) and oxidized low-density lipoprotein (ox-LDL). The original western blot (WB) images are organized in Supplementary Western Blot

    Journal: Frontiers in Bioscience-Landmark

    Article Title: Growth Differentiation Factor 15 Inhibits Cardiac Fibrosis, Oxidative Stress, Inflammation, and Apoptosis in a Rat Model of Heart Failure with Preserved Ejection Fraction

    doi: 10.31083/fbl26857

    Figure Lengend Snippet: Fig. 4. GDF15 decreased oxidative stress in HFpEF. Expression of oxidative stress-associated biomarkers, Myeloperoxidase (MPO) and oxidized low-density lipoprotein (ox-LDL). The original western blot (WB) images are organized in Supplementary Western Blot

    Article Snippet: The primary antibodies utilized included rabbit anti-GDF15 antibody (1:2000; 27455-1-AP; Proteintech; Wuhan, China), mouse anti-NTproBNP antibody (1:1000; ab13115; abcam; Cambridge, UK), rabbit anti-Collagen I antibody (1:1000; 14695-1-AP; Proteintech; Wuhan, China), rabbit anti-Collagen III antibody (1:300; 22734-1-AP; Proteintech; Wuhan, China), rabbit anti-α-smooth muscle actin (α-SMA) antibody (1:1000; 14395-1-AP; Proteintech; Wuhan, China), rabbit anti-Myeloperoxidase (MPO) antibody (1:1000; 22225- 1-AP; Proteintech; Wuhan, China), rabbit anti-oxidized low-density lipoprotein (ox-LDL) antibody (1:500; Ys1698R; YaJi Biotechnology Co., Ltd; Shanghai, China), rabbit anti-Interleukin-8 (IL8) antibody (1:500; 27095-1- AP; Proteintech; Wuhan, China), rabbit anti-Interleukin-6 (IL6) antibody (1:500; 21865-1-AP; Proteintech; Wuhan, China), rabbit anti-Tumor Necrosis Factor α (TNFα) antibody (1:500; 17590-1-AP; Proteintech; Wuhan, China), rabbit anti-Interleukin-1 beta (IL-1β) antibody (1:500; 16806-1-AP; Proteintech; Wuhan, China), rabbit antiCleaved Caspase-3 antibody (1:1000; 66470-2-Ig; Proteintech; Wuhan, China), and rabbit anti-BCL2-associated X (Bax) antibody (1:2000; 50599-2-Ig; Proteintech; Wuhan, China).

    Techniques: Expressing, Western Blot

    Fig. 5. GDF15 reduces cardiac inflammatory responses in HFpEF. Expression of inflammation-associated biomarkers, Interleukin-

    Journal: Frontiers in Bioscience-Landmark

    Article Title: Growth Differentiation Factor 15 Inhibits Cardiac Fibrosis, Oxidative Stress, Inflammation, and Apoptosis in a Rat Model of Heart Failure with Preserved Ejection Fraction

    doi: 10.31083/fbl26857

    Figure Lengend Snippet: Fig. 5. GDF15 reduces cardiac inflammatory responses in HFpEF. Expression of inflammation-associated biomarkers, Interleukin-

    Article Snippet: The primary antibodies utilized included rabbit anti-GDF15 antibody (1:2000; 27455-1-AP; Proteintech; Wuhan, China), mouse anti-NTproBNP antibody (1:1000; ab13115; abcam; Cambridge, UK), rabbit anti-Collagen I antibody (1:1000; 14695-1-AP; Proteintech; Wuhan, China), rabbit anti-Collagen III antibody (1:300; 22734-1-AP; Proteintech; Wuhan, China), rabbit anti-α-smooth muscle actin (α-SMA) antibody (1:1000; 14395-1-AP; Proteintech; Wuhan, China), rabbit anti-Myeloperoxidase (MPO) antibody (1:1000; 22225- 1-AP; Proteintech; Wuhan, China), rabbit anti-oxidized low-density lipoprotein (ox-LDL) antibody (1:500; Ys1698R; YaJi Biotechnology Co., Ltd; Shanghai, China), rabbit anti-Interleukin-8 (IL8) antibody (1:500; 27095-1- AP; Proteintech; Wuhan, China), rabbit anti-Interleukin-6 (IL6) antibody (1:500; 21865-1-AP; Proteintech; Wuhan, China), rabbit anti-Tumor Necrosis Factor α (TNFα) antibody (1:500; 17590-1-AP; Proteintech; Wuhan, China), rabbit anti-Interleukin-1 beta (IL-1β) antibody (1:500; 16806-1-AP; Proteintech; Wuhan, China), rabbit antiCleaved Caspase-3 antibody (1:1000; 66470-2-Ig; Proteintech; Wuhan, China), and rabbit anti-BCL2-associated X (Bax) antibody (1:2000; 50599-2-Ig; Proteintech; Wuhan, China).

    Techniques: Expressing

    Fig. 6. GDF15 suppresses apoptosis in cardiomyocytes in HFpEF. (A) Representative photographs of TUNEL staining in heart

    Journal: Frontiers in Bioscience-Landmark

    Article Title: Growth Differentiation Factor 15 Inhibits Cardiac Fibrosis, Oxidative Stress, Inflammation, and Apoptosis in a Rat Model of Heart Failure with Preserved Ejection Fraction

    doi: 10.31083/fbl26857

    Figure Lengend Snippet: Fig. 6. GDF15 suppresses apoptosis in cardiomyocytes in HFpEF. (A) Representative photographs of TUNEL staining in heart

    Article Snippet: The primary antibodies utilized included rabbit anti-GDF15 antibody (1:2000; 27455-1-AP; Proteintech; Wuhan, China), mouse anti-NTproBNP antibody (1:1000; ab13115; abcam; Cambridge, UK), rabbit anti-Collagen I antibody (1:1000; 14695-1-AP; Proteintech; Wuhan, China), rabbit anti-Collagen III antibody (1:300; 22734-1-AP; Proteintech; Wuhan, China), rabbit anti-α-smooth muscle actin (α-SMA) antibody (1:1000; 14395-1-AP; Proteintech; Wuhan, China), rabbit anti-Myeloperoxidase (MPO) antibody (1:1000; 22225- 1-AP; Proteintech; Wuhan, China), rabbit anti-oxidized low-density lipoprotein (ox-LDL) antibody (1:500; Ys1698R; YaJi Biotechnology Co., Ltd; Shanghai, China), rabbit anti-Interleukin-8 (IL8) antibody (1:500; 27095-1- AP; Proteintech; Wuhan, China), rabbit anti-Interleukin-6 (IL6) antibody (1:500; 21865-1-AP; Proteintech; Wuhan, China), rabbit anti-Tumor Necrosis Factor α (TNFα) antibody (1:500; 17590-1-AP; Proteintech; Wuhan, China), rabbit anti-Interleukin-1 beta (IL-1β) antibody (1:500; 16806-1-AP; Proteintech; Wuhan, China), rabbit antiCleaved Caspase-3 antibody (1:1000; 66470-2-Ig; Proteintech; Wuhan, China), and rabbit anti-BCL2-associated X (Bax) antibody (1:2000; 50599-2-Ig; Proteintech; Wuhan, China).

    Techniques: TUNEL Assay, Staining

    Reported functions of MIC-1 in several cancer types

    Journal: Cancer Cell International

    Article Title: Clinical usefulness and acceleratory effect of macrophage inhibitory cytokine-1 on biliary tract cancer: an experimental biomarker analysis

    doi: 10.1186/s12935-022-02668-x

    Figure Lengend Snippet: Reported functions of MIC-1 in several cancer types

    Article Snippet: After electrophoresis, the proteins were transferred to a PVDF membrane; the membrane was incubated with the primary antibody against GDF15 (1:1000, rabbit monoclonal antibody, no. 8479; Cell Signaling Technology, Beverly, MA, USA) at 4 °C overnight, anti-rabbit IgG secondary antibody (1:1000, no. 7074; Cell Signaling Technology) for one hour at room temperature, and an anti-β-actin polyclonal antibody (MBL, Tokyo, Japan) for one hour at room temperature.

    Techniques: In Vitro, In Vivo, Migration